Journal: International Journal of Molecular Sciences

Article Title: Silencing DTX3L Inhibits the Progression of Cervical Carcinoma by Regulating PI3K/AKT/mTOR Signaling Pathway

doi: 10.3390/ijms24010861

Figure Lengend Snippet: Knockdown of DTX3L promoted HeLa and SiHa cell apoptosis. ( A , B ) Immunofluorescence analysis of c-Caspase-3 expression in HeLa and SiHa cells treated with shDTX3L or shCtrl. The quantified result is shown in the right pane. ( C ) The apoptosis ability of HeLa and SiHa cells was detected by flow cytometry, and the percentage of early apoptotic (PE-A+/7-AAD−) and late apoptotic (PE-A+/7-AAD−) cells were combined for analysis. The quantified result is shown in the right panel. ( D ) Protein expression of Bcl-2, P53, Casepased-3 and c-Casepased-3 in HeLa and SiHa cells transfected with shDTX3L or shCtrl were determined by Western blot analysis. The bar chart in the right shows the ratio of Bcl-2, P53, Casepased-3 and c-Casepased-3 to Actin by densitometry. * p < 0.05; ** p < 0.01.

Article Snippet: Then, the cells were incubated with primary antibodies (c-Caspase-3, CST, #8172, 1:200) at 4 °C overnight and incubated with the appropriate secondary antibodies for 1 h at room temperature.

Techniques: Knockdown, Immunofluorescence, Expressing, Flow Cytometry, Transfection, Western Blot

Journal: The Journal of Pathology

Article Title: Loss of N‐WASP drives early progression in an Apc model of intestinal tumourigenesis

doi: 10.1002/path.5086

Figure Lengend Snippet: Details of the antibodies used for immunohistochemistry

Article Snippet: Caspase 3 (ASP‐175) rabbit monoclonal , Cell Signaling, Leiden, The Netherlands , 9661 , 1:50 , PT Module pH 6 sodium citrate (98 °C).

Techniques:

Journal: Life Science Alliance

Article Title: SMN promotes mitochondrial metabolic maturation during myogenesis by regulating the MYOD-miRNA axis

doi: 10.26508/lsa.202201457

Figure Lengend Snippet: Resources and reagents used in this study.

Article Snippet: Rabbit monoclonal cleaved caspase-3 (Asp175) (D3E9) rabbit (Alexa Fluor 647 conjugate) , Cell Signaling Technology , Cat# 9602S; RRID: AB_2687881.

Techniques: Transduction, Control, Negative Control, Virus, Expressing, Construct, Recombinant, Flow Cytometry, Isolation, Software